RenardV1, Main, Exploration, bibRecord, 000786

Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis

Identifieur interne : 000786 ( Main/Exploration ); précédent : 000785; suivant : 000787

Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis

Auteurs : Morten Tryland [Norvège] ; Kjell Handeland [Norvège] ; Anna-Marie Bratberg [Norvège] ; Inge-Tom Solbakk [Norvège] ; Antti Oksanen [Finlande]

Source :

Acta Veterinaria Scandinavica [ 0044-605X ] ; 2006.

RBID : PMC:1553463

Abstract

To assist in evaluating serological test results from dead animals, 10 silver foxes (Vulpes vulpes) and 10 blue foxes (Alopex lagopus), 6 of each species previously vaccinated against and all challenged with Microsporum canis, were blood sampled and euthanased. Fox carcasses were stored at +10°C, and autopsy was performed on Days 0, 2, 4, 7, and 11 post mortem during which samples from blood and/or body fluid from the thoracic cavity were collected. Antibodies against M. canis were measured in an enzyme-linked immunosorbent assay (ELISA) as absorbance values (optical density; OD). To assess the degradation of antibodies, the ratio between post mortem and ante mortem absorbance was calculated. The mean absorbance from samples collected during autopsy was generally lower than from samples from live animals. In blood samples, this difference increased significantly with time (P = 0.04), while in body fluid samples the difference decreased (not significant; P = 0.18). We suggest that a positive serological result from testing blood or body fluid of a dead animal may be regarded as valuable, although specific prevalences obtained by screening populations based on this type of material may represent an under-estimation of the true antibody prevalence. Negative serological test results based on material from carcasses may be less conclusive, taken into account the general degradation processes in decaying carcasses, also involving immunoglobulin proteins.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1553463

DOI: 10.1186/1751-0147-48-10
PubMed: 16987389
PubMed Central: 1553463

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against <italic>Microsporum canis</italic>
</title>
<author><name sortKey="Tryland, Morten" sort="Tryland, Morten" uniqKey="Tryland M" first="Morten" last="Tryland">Morten Tryland</name>
<affiliation wicri:level="1"><nlm:aff id="I1">The Norwegian School of Veterinary Science, Section of Arctic Veterinary Medicine, P.O. Box 6204, N-9292 Tromsø, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>The Norwegian School of Veterinary Science, Section of Arctic Veterinary Medicine, P.O. Box 6204, N-9292 Tromsø</wicri:regionArea>
<wicri:noRegion>N-9292 Tromsø</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Handeland, Kjell" sort="Handeland, Kjell" uniqKey="Handeland K" first="Kjell" last="Handeland">Kjell Handeland</name>
<affiliation wicri:level="3"><nlm:aff id="I2">National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bratberg, Anna Marie" sort="Bratberg, Anna Marie" uniqKey="Bratberg A" first="Anna-Marie" last="Bratberg">Anna-Marie Bratberg</name>
<affiliation wicri:level="3"><nlm:aff id="I2">National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Solbakk, Inge Tom" sort="Solbakk, Inge Tom" uniqKey="Solbakk I" first="Inge-Tom" last="Solbakk">Inge-Tom Solbakk</name>
<affiliation wicri:level="3"><nlm:aff id="I2">National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Oksanen, Antti" sort="Oksanen, Antti" uniqKey="Oksanen A" first="Antti" last="Oksanen">Antti Oksanen</name>
<affiliation wicri:level="1"><nlm:aff id="I3">National Veterinary and Food Research Institute EELA, Oulu Regional Unit, P.O.Box 517, FIN-90101 Oulu, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea>National Veterinary and Food Research Institute EELA, Oulu Regional Unit, P.O.Box 517, FIN-90101 Oulu</wicri:regionArea>
<wicri:noRegion>FIN-90101 Oulu</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">16987389</idno>
<idno type="pmc">1553463</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1553463</idno>
<idno type="RBID">PMC:1553463</idno>
<idno type="doi">10.1186/1751-0147-48-10</idno>
<date when="2006">2006</date>
<idno type="wicri:Area/Pmc/Corpus">000033</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000033</idno>
<idno type="wicri:Area/Pmc/Curation">000033</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000033</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000076</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000076</idno>
<idno type="wicri:Area/Ncbi/Merge">000160</idno>
<idno type="wicri:Area/Ncbi/Curation">000160</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000160</idno>
<idno type="wicri:doubleKey">0044-605X:2006:Tryland M:persistence:of:antibodies</idno>
<idno type="wicri:Area/Main/Merge">000804</idno>
<idno type="wicri:Area/Main/Curation">000786</idno>
<idno type="wicri:Area/Main/Exploration">000786</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against <italic>Microsporum canis</italic>
</title>
<author><name sortKey="Tryland, Morten" sort="Tryland, Morten" uniqKey="Tryland M" first="Morten" last="Tryland">Morten Tryland</name>
<affiliation wicri:level="1"><nlm:aff id="I1">The Norwegian School of Veterinary Science, Section of Arctic Veterinary Medicine, P.O. Box 6204, N-9292 Tromsø, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>The Norwegian School of Veterinary Science, Section of Arctic Veterinary Medicine, P.O. Box 6204, N-9292 Tromsø</wicri:regionArea>
<wicri:noRegion>N-9292 Tromsø</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Handeland, Kjell" sort="Handeland, Kjell" uniqKey="Handeland K" first="Kjell" last="Handeland">Kjell Handeland</name>
<affiliation wicri:level="3"><nlm:aff id="I2">National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bratberg, Anna Marie" sort="Bratberg, Anna Marie" uniqKey="Bratberg A" first="Anna-Marie" last="Bratberg">Anna-Marie Bratberg</name>
<affiliation wicri:level="3"><nlm:aff id="I2">National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Solbakk, Inge Tom" sort="Solbakk, Inge Tom" uniqKey="Solbakk I" first="Inge-Tom" last="Solbakk">Inge-Tom Solbakk</name>
<affiliation wicri:level="3"><nlm:aff id="I2">National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway</nlm:aff>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo</wicri:regionArea>
<placeName><settlement type="city">Oslo</settlement>
<region nuts="2">Østlandet</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Oksanen, Antti" sort="Oksanen, Antti" uniqKey="Oksanen A" first="Antti" last="Oksanen">Antti Oksanen</name>
<affiliation wicri:level="1"><nlm:aff id="I3">National Veterinary and Food Research Institute EELA, Oulu Regional Unit, P.O.Box 517, FIN-90101 Oulu, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea>National Veterinary and Food Research Institute EELA, Oulu Regional Unit, P.O.Box 517, FIN-90101 Oulu</wicri:regionArea>
<wicri:noRegion>FIN-90101 Oulu</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Acta Veterinaria Scandinavica</title>
<idno type="ISSN">0044-605X</idno>
<idno type="eISSN">1751-0147</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>To assist in evaluating serological test results from dead animals, 10 silver foxes (<italic>Vulpes vulpes</italic>
) and 10 blue foxes (<italic>Alopex lagopus</italic>
), 6 of each species previously vaccinated against and all challenged with <italic>Microsporum canis</italic>
, were blood sampled and euthanased. Fox carcasses were stored at +10°C, and autopsy was performed on Days 0, 2, 4, 7, and 11 <italic>post mortem </italic>
during which samples from blood and/or body fluid from the thoracic cavity were collected. Antibodies against <italic>M. canis </italic>
were measured in an enzyme-linked immunosorbent assay (ELISA) as absorbance values (optical density; OD). To assess the degradation of antibodies, the ratio between <italic>post mortem </italic>
and <italic>ante mortem </italic>
absorbance was calculated. The mean absorbance from samples collected during autopsy was generally lower than from samples from live animals. In blood samples, this difference increased significantly with time (<italic>P </italic>
= 0.04), while in body fluid samples the difference decreased (not significant; <italic>P </italic>
= 0.18). We suggest that a positive serological result from testing blood or body fluid of a dead animal may be regarded as valuable, although specific prevalences obtained by screening populations based on this type of material may represent an under-estimation of the true antibody prevalence. Negative serological test results based on material from carcasses may be less conclusive, taken into account the general degradation processes in decaying carcasses, also involving immunoglobulin proteins.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Finlande</li>
<li>Norvège</li>
</country>
<region><li>Østlandet</li>
</region>
<settlement><li>Oslo</li>
</settlement>
</list>
<tree><country name="Norvège"><noRegion><name sortKey="Tryland, Morten" sort="Tryland, Morten" uniqKey="Tryland M" first="Morten" last="Tryland">Morten Tryland</name>
</noRegion>
<name sortKey="Bratberg, Anna Marie" sort="Bratberg, Anna Marie" uniqKey="Bratberg A" first="Anna-Marie" last="Bratberg">Anna-Marie Bratberg</name>
<name sortKey="Handeland, Kjell" sort="Handeland, Kjell" uniqKey="Handeland K" first="Kjell" last="Handeland">Kjell Handeland</name>
<name sortKey="Solbakk, Inge Tom" sort="Solbakk, Inge Tom" uniqKey="Solbakk I" first="Inge-Tom" last="Solbakk">Inge-Tom Solbakk</name>
</country>
<country name="Finlande"><noRegion><name sortKey="Oksanen, Antti" sort="Oksanen, Antti" uniqKey="Oksanen A" first="Antti" last="Oksanen">Antti Oksanen</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000786 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000786 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    RenardV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:1553463
   |texte=   Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:16987389" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a RenardV1

This area was generated with Dilib version V0.6.27.
Data generation: Tue Mar 28 00:55:51 2017. Site generation: Thu Jan 4 16:57:14 2024

Serveur d'exploration sur le renard

Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis

Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le renard
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.